Illumina Library Preparation Costs
Table 1. Illumina library preparation costs (prices valid for libraries prepared AND sequenced at GGBC ONLY*)
| Illumina Compatible Library Type | UGA Fee | UGA Fee | Non-UGA Fee | Non-UGA Fee | Commercial Fee | Commercial Fee |
|---|---|---|---|---|---|---|
| (Price per library for up to 12) | (Price for each additional library) | (Price per library for up to 12) | (Price for each additional library) | (Price per library for up to 12) | (Price for each additional library) | |
| RNA libraries | ||||||
| NGS Stranded or Unstranded RNA (see Table 2) | $132 | $112 | $156 | $133 | $165 | $140 |
| NGS Ribo-Depleted Stranded or Unstranded RNA Library | $195 | $195 | $231 | $231 | $244 | $244 |
| NGS Small RNA | $115 | $92 | $136 | $109 | $144 | $115 |
| cDNA synthesis | ||||||
| cDNA 2nd strand synthesis only (1 to 24 samples per plate) | $447 | $528 | $559 | |||
| cDNA 2nd strand synthesis only (25 to 48 samples per plate) | $894 | $1,055 | $1,118 | |||
| cDNA 2nd strand synthesis only (49 to 72 samples per plate) | $1,341 | $1,583 | $1,677 | |||
| cDNA 2nd strand synthesis only (73 to 96 samples per plate) | $1,788 | $2,110 | $2,235 | |||
| Double stranded cDNA synthesis (1 to 24 samples per plate) | $598 | $706 | $748 | |||
| Double stranded cDNA synthesis (25 to 48 samples per plate) | $1,195 | $1,411 | $1,494 | |||
| Double stranded cDNA synthesis (49 to 72 samples per plate) | $1,793 | $2,116 | $2,242 | |||
| Double stranded cDNA synthesis (73 to 96 samples per plate) | $2,390 | $2,821 | $2,988 | |||
| cDNA from low input RNA – SMRT technology (Input 1-2 ng total RNA) | $159 | $159 | $188 | $188 | $239 | $239 |
| DNA libraries | ||||||
| NGS DNA ** (see Table 2) | $132 | $95 | $156 | $113 | $165 | $119 |
| NGS DNA-PCR Free Library Prep (1-12 samples) | $128 | $152 | $160 | |||
| NGS DNA-PCR Free Library Prep (13 or more samples) | $92 | $109 | $115 | |||
| Enzymatic Methyl-Seq | $96 | $96 | $114 | $114 | $120 | $120 |
| Zymo-Seq RRBS | $120 | $120 | $142 | $142 | $150 | $150 |
*Prices are significantly higher If libraries are to be prepared at GGBC but sequenced elsewhere. Please inquire for pricing.
Table 2. Illumina compatible library preparation reduced flat fees for samples submitted in a 96-well plate
| llumina Compatible Library Type (submitted in 96 well plate) | UGA Fee | Non-UGA Fee | Commercial Fee |
|---|---|---|---|
| NGS Stranded or Unstranded RNA | $6,115 | $7,216 | $7,644 |
| TruSeq Stranded Total RNA Gold (Human/Rat/Mouse) (up to 48 samples per plate) | $8,810 | 10,396 | $11,013 |
| TruSeq Stranded Total RNA Gold (Human/Rat/Mouse) (49-96 samples per plate) | $15,537 | $18,334 | $19,422 |
| QIAseq FastSelect Ribo-depletion 5S/16S/23S (96-well plate flat rate) | $5,125 | $6,048 | $6,407 |
| NGS DNA-PCR free | $7,639 | $9,015 | $9,549 |
| NGS DNA ** | $7,830 | $9,240 | $9,788 |
| Nextera Flex for Enrichment (up to 48 samples per plate) | $7,019 | $8,283 | $8,774 |
| Nextera Flex for Enrichment (49-96 samples per plate) | $11,697 | $13,803 | $14,662 |
| Modified Amplicons (16S/ITS/Custom)(up to 48 samples per plate) | $457 | $540 | $572 |
| Modified Amplicons (16S/ITS/Custom)(49 to 96 samples per plate) | $681 | $804 | $852 |
Extra charges will be incurred if sample(s) do not pass QC and are resubmitted. Any extra sample(s) above 96 will be charged according to Table 1.
**Illumina has changed the bead component in their library preparation kits and the Nextera DNA Flex products are now called Illumina DNA prep.