DNA Size Selection

The Sage Electrophoretic Lateral Fractionator, SageELF, is a gel electrophoresis system for automated separation and elution of DNA and proteins in 12 consecutive fractions of different sizes.

For DNA fractionation, three gel cassette types are available with the following target ranges:

• 100 bp to 2.3 kb on 2% agarose gel (call GGBC to check availability)
• 1 kb to 18 kb on 0.75% agarose gel (in stock)
• 10 kb to 40 kb on 0.75% agarose gel (in stock)

Submit 100 ng to 5 µg of DNA in a maximum volume of 30 µL TE or nuclease free water. Samples should be free of ligases or polymerases and have low salt concentrations.

For protein fractionation, two gel cassettes types are available with the following target ranges:

• 10 to 150 kDa on 5% agarose gel (call GGBC to check availability)
• 10 to 300 kDa on 3% agarose gel (call GGBC to check availability)

More information on the SageELF can be found on the Sage Science website.

Contact for Financial Inquiries and Quote Requests

Please email Kim and Elizabeth at ggbc@uga.edu, for financial inquiries or to request a quote. Be as specific as possible, so that they can more quickly assist you.

Table 1. Price for DNA size selection on the SageELF (one sample per cassette)

Cassette Type and Size Range	UGA Fee	Non-UGA Fee	Commercial Fee
2% Native Agarose Cassette for DNA, 100-2300 bp	$116.00	$137.00	$145.00
0.75% Native Agarose Cassette for DNA, 1-18 kb	$116.00	$137.00	$145.00
0.75% Native Agarose Cassette for DNA, 10-40 kb	$116.00	$137.00	$145.00
3% SDS-Agarose Cassette for Protein, 10-300 kDa	$144.00	$170.00	$180.00
5% SDS-Agarose Cassette for Protein, 10-150 kDa	$144.00	$170.00	$180.00

Quality controls using the Qubit and Fragment Analyzer will be performed on eluted fractions (additional costs may be incurred).