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MiSeq and NextSeq Flow Cells

Table 4. MiSeq flow cells

Run TypeExpected single/paired-end reads passing filterExpected OutputUGA FeeNon-UGA FeeCommercial Fee
v2 Flow Cell
300 cycles12-15M / 24-30M4.5-5.1 Gb$1,771$2,090$2,214


Table 5. NextSeq 2000 flow cells

Run TypeExpected single/paired-end reads passing filterExpected OutputUGA FeeNon-UGA
Fee
Commercial
Fee
P1 Flow Cell
100 cycles100M / 200M10 Gb$1,232.00$1,454.00$1,540.00
300 cycles100M / 200M30 Gb$1,540.00$1,818.00$1,925.00
600 cycles100M / 200M60 Gb$2,113.00$2,494.00$2,642.00
P2 Flow Cell
100 cycles400M / 800M40 Gb$1,721.00$2,031.00$2,152.00
200 cycles400M / 800M80 Gb$2,850.00$3,363.00$3,563.00
300 cycles400M / 800M120 Gb$3,636.00$4,291.00$4,545.00
600 cycles300M / 600M180 Gb$3,920.00$4,626.00$4,900.00
P3 Flow Cell
50 cycles1.2B / 2.4 B60 Gb$2,471.00$2,916.00$3,089.00
100 cycles1.2B / 2.4 B120 Gb$3,374.00$3,982.00$4,218.00
200 cycles1.2B / 2.4 B240 Gb$4,504.00$5,315.00$5,630.00
300 cycles1.2B / 2.4 B360 Gb$5,859.00$6,914.00$7,324.00

Library Pooling and Quality Control

Table 3. Library pooling and Quality control

Service DescriptionUGA FeeNon-UGA FeeCommercial Fee
Library Pooling up to 2-24 samples$60$71$75
Library Pooling up to 25-48 samples$105$124$132
Library Pooling up to 49-96 samples$162$192$203
Library Pooling up to 97-144 samples$234$277$293
Library Pooling up to 145-192 samples$306$362$383
Library Pooling up to 193-288 samples$436$515$545
Library Pooling up to 289-384 samples$565$667$707
Pre-Sequencing QC (Qubit, FA, Kapa)$120$142$150
Additional Qubit quantification (per sample)$5.00$5.90$6.25
Additional Fragment Analyzer: High sensitivity NGS fragment analysis (per sample)$8.20$9.70$10.25
Additional Kapa qPCR (per sample)$49.00$57.85$61.25

Illumina Library Preparation Costs

Table 1. Illumina library preparation costs (prices valid for libraries prepared AND sequenced at GGBC ONLY*)

Illumina Compatible
Library Type
UGA Fee
UGA Fee
Non-UGA FeeNon-UGA FeeCommercial FeeCommercial Fee
(Price per library for up to 12)(Price for each additional library)(Price per library for up to 12)(Price for each additional library)(Price per library for up to 12)(Price for each additional library)
RNA libraries
NGS Stranded or Unstranded RNA
(see Table 2)
$132$112$156$133$165$140
NGS Ribo-Depleted Stranded or Unstranded RNA Library $195$195$231$231$244$244
NGS Small RNA$115$92$136$109$144$115
cDNA synthesis
cDNA 2nd strand synthesis only (1 to 24 samples per plate)$447$528$559
cDNA 2nd strand synthesis only (25 to 48 samples per plate)$894$1,055$1,118
cDNA 2nd strand synthesis only (49 to 72 samples per plate)$1,341$1,583$1,677
cDNA 2nd strand synthesis only (73 to 96 samples per plate)$1,788$2,110$2,235
Double stranded cDNA synthesis (1 to 24 samples per plate)$598$706$748
Double stranded cDNA synthesis (25 to 48 samples per plate)$1,195$1,411$1,494
Double stranded cDNA synthesis (49 to 72 samples per plate)$1,793$2,116$2,242
Double stranded cDNA synthesis (73 to 96 samples per plate)$2,390$2,821$2,988
cDNA from low input
RNA – SMRT technology
(Input 1-2 ng total RNA)
$159$159$188$188$239$239
DNA libraries
NGS DNA **
(see Table 2)
$132$95$156$113$165$119
NGS DNA-PCR Free Library Prep (1-12 samples)$128$152$160
NGS DNA-PCR Free Library Prep (13 or more samples)$92$109$115
Enzymatic Methyl-Seq$96$96$114$114$120$120
Zymo-Seq RRBS$120$120$142$142$150$150

*Prices are significantly higher If libraries are to be prepared at GGBC but sequenced elsewhere. Please inquire for pricing.

Table 2. Illumina compatible library preparation reduced flat fees for samples submitted in a 96-well plate

llumina Compatible Library Type (submitted in 96 well plate)UGA FeeNon-UGA FeeCommercial Fee
NGS Stranded or Unstranded RNA$6,115$7,216$7,644
TruSeq Stranded Total RNA Gold (Human/Rat/Mouse) (up to 48 samples per plate)$8,81010,396$11,013
TruSeq Stranded Total RNA Gold (Human/Rat/Mouse) (49-96 samples per plate)$15,537$18,334$19,422
QIAseq FastSelect Ribo-depletion 5S/16S/23S (96-well plate flat rate)$5,125$6,048$6,407
NGS DNA-PCR free$7,639$9,015$9,549
NGS DNA **$7,830$9,240$9,788
Nextera Flex for Enrichment (up to 48 samples per plate)$7,019$8,283$8,774
Nextera Flex for Enrichment (49-96 samples per plate)$11,697$13,803$14,662
Modified Amplicons (16S/ITS/Custom)(up to 48 samples per plate)$457$540$572
Modified Amplicons (16S/ITS/Custom)(49 to 96 samples per plate)$681$804$852

Extra charges will be incurred if sample(s) do not pass QC and are resubmitted. Any extra sample(s) above 96 will be charged according to Table 1.

**Illumina has changed the bead component in their library preparation kits and the Nextera DNA Flex products are now called Illumina DNA prep.

Immune Profiling

Table 7. 10x Genomics Immune Profiling 5’ GEX + V(D)J libraries preparation fees

Number of Sample(s)UGA FeeNon-UGA FeeCommercial Fee
1$ 3,084.00$ 3,640.00$ 3,855.00
2$ 5,346.00$ 6,309.00$ 6,683.00
3$ 7,608.00$ 8,978.00$ 9,510.00
4$ 9,871.00$ 11,648.00$ 12,339.00
5$ 12,408.00$ 14,642.00$ 15,510.00
6$ 14,670.00$ 17,311.00$ 18,338.00
7$ 16,933.00$ 19,981.00$ 21,167.00
8$ 19,195.00$ 22,651.00$ 23,994.00

Table 8. Suggested Illumina sequencing run type and associated fees for Immune Profiling 5’ GEX and V(D)J

Service Description(s)UGA FeeNon-UGA FeeCommercial Fee
Library Pooling up to 2-24 samples*$60$71$75
Pre-Sequencing QC (Qubit, FA, Kapa)(per run)$120.00$142.00$150.00
NextSeq2000 (100 Cycles) flow cell(P2)$2,013.00$2,376.00$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)$3,398.00$4,010.00$4,248.00
NextSeq2000 (100 Cycles) flow cell(P3)$4,040.00$4,768.00$5,050.00
NextSeq2000 (200 Cycles) flow cell(P3)$5,426.00$6,403.00$6,783.00

Each Immune Profiling sample produces two libraries: 5’ GEX and V(D)J. Each library requires a separate Nextseq2000 sequencing run.

Single Cell Multiome ATAC + Gene Expression

Table 5. 10x Genomics Single Cell Multiome ATAC + Gene Expression libraries preparation fees

Number of Sample(s)UGA FeeNon-UGA FeeCommercial Fee
1$4,345.00$5.128.00$5,432.00
2$ 7,728.00$ 9,120.00$ 9,660.00
3$11,111.00$ 13,111.00$ 13,889.00
4$ 14,493.00$ 17,102.00$ 18,117.00
5$ 18,151.00$ 21,419.00$ 22,689.00
6$ 21,534.00$ 25,411.00$ 26,918.00
7$ 24,917.00$ 29,403.00$ 31,147.00
8$ 28,299.00$ 33,393.00$ 35,374.00

Table 6. Suggested Illumina sequencing run type and associated fees for Single Cell Multiome ATAC + Gene Expression libraries

Service Description(s)UGA FeeNon-UGA FeeCommercial Fee
Library Pooling up to 2-24 samples*$60$71$75
Pre-Sequencing QC (Qubit, FA, Kapa)(per run)$120.00$142.00$150.00
NextSeq2000 (100 Cycles) flow cell(P2)$2,013.00$2,376.00$2,517.00
NextSeq2000 (200 Cycles) flow cell(P2)$3,398.00$4,010.00 $4,248.00
NextSeq2000 (100 Cycles) flow cell(P3)$4,040.00$4,768.00$5,050.00
NextSeq2000 (200 Cycles) flow cell(P3)$5,426.00$6,403.00$6,783.00

Each Multiome sample produces two libraries: ATAC and 3’RNAseq. Each library requires a separate NextSeq2000 sequencing run.

scATAC-seq

Table 3. 10x Genomics scATAC-seq library preparation fees

Number of Sample(s)UGA FeeNon-UGA FeeCommercial Fee
1$2,759.00$3,256.00$3,449.00
2$4,637.00$5,472.00$5,797.00
3$6,515.00$7,688.00$8,144.00
4$8,393.00$9,904.00$10,492.00
5$10,537.00$12,434.00$13,172.00
6$12,415.00$14,650.00$15,519.00
7$14,293.00$16,866.00$17,867.00
8$16,171.00$19,082.00$20,214.00

Table 4. Suggested Illumina sequencing run type and associated fees for scATAC-seq libraries

Service Description(s)UGA FeeNon-UGA FeeCommercial Fee
Library Pooling up to 2-24 samples*$60$71$75
Pre-Sequencing QC (Qubit, FA, Kapa)$120.00$142.00$150.00
NextSeq500 (150 Cycles) High Output flow cell $3,899.00$4,601.00$4,874.00
NextSeq500 (75 Cycles) High Output flow cell $2,253.00$2,659.00$2,817.00
NextSeq2000 (100 Cycles) flow cell (P2)$2,013.00$2,376.00$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)$3,398.00$4,010.00$4,248.00
NextSeq2000 (100 Cycles) flow cell (P3)$4,040.00$4,768.00 $5,050.00
NextSeq2000 (200 Cycles) flow cell (P3)$5,426.00$6,403.00$6,783.00

Single Cell/Nuclei RNA seq

Table 1. 10x Genomics single cell/nuclei RNA seq library preparation fees

Number of Sample(s)UGA FeeNon-UGA FeeCommercial Fee
1$2,758.00$3,255.00$3,448.00
2$4,662.00$5,502.00$5,828.00
3$6,565.00$7,747.00$8,207.00
4$8,469.00$9,994.00$10,587.00
5$10,622.00$12,534.00$13,278.00
6$12,526.00$14,781.00$15,658.00
7$14,429.00$17,027.00$18,037.00
8$16,333.00$19,273.00$20,417.00

Table 2. Suggested Illumina sequencing run type and associated fees for single cell/nuclei RNAseq libraries

Service Description(s)UGA FeeNon-UGA FeeCommercial Fee
Library Pooling up to 2-24 samples*$60$71$75
Pre-Sequencing QC (Qubit, FA, Kapa)$120.00$142.00$150.00
NextSeq500 (150 Cycles) Mid Output flow cell $1,763.00$2,081.00$2,204.00
NextSeq500 (150 Cycles) High Output flow cell$3,899.00$4,601.00$4,874.00
NextSeq500 (75 Cycles) High Output flow cell$2,253.00$2,659.00$2,817.00
NextSeq2000 (100 Cycles) flow cell (P2)$2,013.00$2,376.00$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)$3,398.00$4,010.00 $4,248.00
NextSeq2000 (100 Cycles) flow cell (P3)$4,040.00$4,768.00 $5,050.00
NextSeq2000 (200 Cycles) flow cell (P3)$5,426.00$6,403.00$6,783.00