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Table 4. MiSeq i100 Plus flow cells

Run Type	Expected single/paired end reads passing filter	Expected Output	UGA Fee	Non-UGA Fee	Commercial Fee
5M Flow Cell
300 cycles	5M/10M	1.5 Gb	$651.00	$769.00	$814.00
600 cycles	5M/10M	3 Gb	$933.00	$1,101.00	$1,167.00
25M Flow Cell
100 cycles	25M/50M	2.5 Gb	$861.00	$1,016.00	$1,077.00
300 cycles	25M/50M	7.5 Gb	$1,293.00	$1,526.00	$1,617.00
600 cycles	25M/50M	15 Gb	$1,640.00	$1,936.00	$2,050.00
1000 cycles	25M/50M	25 Gb	$2,390.00	$2,821.00	$2,988.00
50M Flow Cell
100 cycles	50M/100M	5 Gb	$1,112.00	$1,313.00	$1,390.00
300 cycles	50M/100M	15 Gb	$1,546.00	$1,825.00	$1,933.00
600 cycles	50M/100M	30 Gb	$2,081.00	$2,456.00	$2,602.00
100M Flow Cell
100 cycles	100M/200M	10Gb	$1,422.00	$1,678.00	$1,778.00
300 cycles	100M/200M	30 Gb	$1,981.00	$2,338.00	$2,477.00

Table 5. NextSeq 2000 flow cells

Run Type	Expected single/paired-end reads passing filter	Expected Output	UGA Fee	Non-UGA Fee	Commercial Fee
P1 Flow Cell
100 cycles	100M / 200M	10 Gb	$1,232.00	$1,454.00	$1,540.00
300 cycles	100M / 200M	30 Gb	$1,540.00	$1,818.00	$1,925.00
600 cycles	100M / 200M	60 Gb	$2,113.00	$2,494.00	$2,642.00
P2 Flow Cell
100 cycles	400M / 800M	40 Gb	$1,721.00	$2,031.00	$2,152.00
200 cycles	400M / 800M	80 Gb	$2,850.00	$3,363.00	$3,563.00
300 cycles	400M / 800M	120 Gb	$3,636.00	$4,291.00	$4,545.00
600 cycles	400M / 800M	240 Gb	$3,920.00	$4,626.00	$4,900.00
P3 Flow Cell
50 cycles	1.2B / 2.4 B	60 Gb	$2,471.00	$2,916.00	$3,089.00
100 cycles	1.2B / 2.4 B	120 Gb	$3,374.00	$3,982.00	$4,218.00
200 cycles	1.2B / 2.4 B	240 Gb	$4,504.00	$5,315.00	$5,630.00
300 cycles	1.2B / 2.4 B	360 Gb	$5,859.00	$6,914.00	$7,324.00
P4 Flow Cell
50 cycles	1.8B/3.6B	90 Gb	$2,448.00	$2,889.00	$3,060.00
100 cycles	1.8B/3.6B	180 Gb	$3,453.00	$4,075.00	$4,317.00
200 cycles	1.8B/3.6B	360 Gb	$4,701.00	$5,548.00	$5,877.00
300 cycles	1.8B/3.6B	540 Gb	$5,693.00	$6,718.00	$7,117.00

Library Pooling and Quality Control

slquinlan on June 6, 2023

Table 3. Library pooling and Quality control

Service Description	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples	$63	$74.50	$78.75
Library Pooling up to 25-48 samples	$110	$130	$138
Library Pooling up to 49-96 samples	$170	$201	$213
Library Pooling up to 97-144 samples	$241	$285	$302
Library Pooling up to 145-192 samples	$268	$317	$335
Library Pooling up to 193-288 samples	$295	$349	$369
Library Pooling up to 289-384 samples	$378	$447	$473
Pre-Sequencing QC (Qubit, FA, Kapa)	$126	$149	$158
Additional Qubit quantification (per sample)	$5.00	$5.90	$6.25
Additional Fragment Analyzer: High sensitivity NGS fragment analysis (per sample)	$8.20	$9.70	$10.25
Additional Kapa qPCR (per sample)	$49.00	$57.85	$61.25

Illumina Library Preparation Costs

slquinlan on June 5, 2023

Table 1. Illumina library preparation costs

Illumina Compatible Library Type	UGA Fee	UGA Fee	Non-UGA Fee	Non-UGA Fee	Commercial Fee	Commercial Fee
	_{(Price per library for up to 12)}	_{(Price for each additional library)}	_{(Price per library for up to 12)}	_{(Price for each additional library)}	_{(Price per library for up to 12)}	_{(Price for each additional library)}
RNA libraries
NGS Stranded (Illumina)	$80	$70	$94.50	$82.75	$100	$87.50
NGS Stranded (Roche KAPA)	$132	$112	$156	$133	$165	$140
NGS Ribo-Depleted Stranded or Unstranded RNA Library	Inquire	Inquire	Inquire	Inquire	Inquire	Inquire
NGS Small RNA	$115	$92	$136	$109	$144	$115
DNA libraries
NGS DNA	$45	$40	$53.10	$47.20	$56.25	$50
NGS DNA-PCR Free Library Prep	$128	$92	$152	$109	$160	$115
Enzymatic Methyl-Seq	$96	$96	$114	$114	$120	$120
Zymo-Seq RRBS	$120	$120	$142	$142	$150	$150

*Prices are significantly higher If libraries are to be prepared at GGBC but sequenced elsewhere. Please inquire for pricing.

Table 2. Illumina compatible library preparation reduced flat fees for samples submitted in a 96-well plate

Illumina Compatible Library Type (submitted in 96 well, full or half plate)	UGA Fee	Non-UGA Fee	Commercial Fee
NGS Stranded RNA Full Plate Illumina Prep Kit	$4,800	$5,664	$6,000
NGS Stranded RNA Half Plate Illumina Prep Kit	$2,640	$3,116	$3,300
NGS Stranded RNA Full Plate: Roche KAPA Kit	$6,115	$7,216	$7,644
NGS Stranded RNA Half Plate: Roche KAPA Kit	$4,322	$5,100	$5,403
QIAseq FastSelect Ribo-depletion Full Plate	$5,125	$6,048	$6,407
QIAseq FastSelect Ribo-depletion Half Plate	$2,819	$3,326	$3,523
NGS DNA-PCR free	$7,639	$9,015	$9,549
NGS DNA Illumina: NGS DNA Library Prep (96-well full plate flat rate)	$1,920	$2,266	$2,400
NGS DNA Illumina: NGS DNA Library Prep (96-well half plate flat rate)	$1,200	$1,416	$1,500
Amplicons (16S/ITS/Custom)(49 to 96 samples per plate)	$949	$1,120	$1,187
Amplicons (16S/ITS/Custom)(up to 48 samples per plate)	$638	$753	$798
Amplicons (16S/ITS/Custom)(up to 24 samples per plate)	$360	$425	$450
Modified Amplicons (16S/ITS/Custom)(49 to 96 samples per plate)	$681	$804	$852
Modified Amplicons (16S/ITS/Custom)(up to 48 samples per plate)	$457	$540	$572
Modified Amplicons (16S/ITS/Custom)(up to 24 samples per plate)	$258	$305	$323

Extra charges will be incurred if sample(s) do not pass QC and are resubmitted. Any extra sample(s) above 96 will be charged according to Table 1.

**Illumina has changed the bead component in their library preparation kits and the Nextera DNA Flex products are now called Illumina DNA prep.

Table 7. 10x Genomics Immune Profiling 5’ GEX + V(D)J libraries preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$ 3,084.00	$ 3,640.00	$ 3,855.00
2	$ 5,346.00	$ 6,309.00	$ 6,683.00
3	$ 7,608.00	$ 8,978.00	$ 9,510.00
4	$ 9,871.00	$ 11,648.00	$ 12,339.00
5	$ 12,408.00	$ 14,642.00	$ 15,510.00
6	$ 14,670.00	$ 17,311.00	$ 18,338.00
7	$ 16,933.00	$ 19,981.00	$ 21,167.00
8	$ 19,195.00	$ 22,651.00	$ 23,994.00

Table 8. Suggested Illumina sequencing run type and associated fees for Immune Profiling 5’ GEX and V(D)J

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)(per run)	$120.00	$142.00	$150.00
NextSeq2000 (100 Cycles) flow cell(P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell(P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell(P3)	$5,426.00	$6,403.00	$6,783.00

Each Immune Profiling sample produces two libraries: 5’ GEX and V(D)J. Each library requires a separate Nextseq2000 sequencing run.

Single Cell Multiome ATAC + Gene Expression

slquinlan on June 1, 2023

Table 5. 10x Genomics Single Cell Multiome ATAC + Gene Expression libraries preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$4,345.00	$5.128.00	$5,432.00
2	$ 7,728.00	$ 9,120.00	$ 9,660.00
3	$11,111.00	$ 13,111.00	$ 13,889.00
4	$ 14,493.00	$ 17,102.00	$ 18,117.00
5	$ 18,151.00	$ 21,419.00	$ 22,689.00
6	$ 21,534.00	$ 25,411.00	$ 26,918.00
7	$ 24,917.00	$ 29,403.00	$ 31,147.00
8	$ 28,299.00	$ 33,393.00	$ 35,374.00

Table 6. Suggested Illumina sequencing run type and associated fees for Single Cell Multiome ATAC + Gene Expression libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)(per run)	$120.00	$142.00	$150.00
NextSeq2000 (100 Cycles) flow cell(P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell(P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell(P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell(P3)	$5,426.00	$6,403.00	$6,783.00

Each Multiome sample produces two libraries: ATAC and 3’RNAseq. Each library requires a separate NextSeq2000 sequencing run.

Table 3. 10x Genomics scATAC-seq library preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$2,759.00	$3,256.00	$3,449.00
2	$4,637.00	$5,472.00	$5,797.00
3	$6,515.00	$7,688.00	$8,144.00
4	$8,393.00	$9,904.00	$10,492.00
5	$10,537.00	$12,434.00	$13,172.00
6	$12,415.00	$14,650.00	$15,519.00
7	$14,293.00	$16,866.00	$17,867.00
8	$16,171.00	$19,082.00	$20,214.00

Table 4. Suggested Illumina sequencing run type and associated fees for scATAC-seq libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)	$120.00	$142.00	$150.00
NextSeq500 (150 Cycles) High Output flow cell	$3,899.00	$4,601.00	$4,874.00
NextSeq500 (75 Cycles) High Output flow cell	$2,253.00	$2,659.00	$2,817.00
NextSeq2000 (100 Cycles) flow cell (P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell (P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell (P3)	$5,426.00	$6,403.00	$6,783.00

Table 1. 10x Genomics single cell/nuclei RNA seq library preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$2,758.00	$3,255.00	$3,448.00
2	$4,662.00	$5,502.00	$5,828.00
3	$6,565.00	$7,747.00	$8,207.00
4	$8,469.00	$9,994.00	$10,587.00
5	$10,622.00	$12,534.00	$13,278.00
6	$12,526.00	$14,781.00	$15,658.00
7	$14,429.00	$17,027.00	$18,037.00
8	$16,333.00	$19,273.00	$20,417.00

Table 2. Suggested Illumina sequencing run type and associated fees for single cell/nuclei RNAseq libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)	$120.00	$142.00	$150.00
NextSeq500 (150 Cycles) Mid Output flow cell	$1,763.00	$2,081.00	$2,204.00
NextSeq500 (150 Cycles) High Output flow cell	$3,899.00	$4,601.00	$4,874.00
NextSeq500 (75 Cycles) High Output flow cell	$2,253.00	$2,659.00	$2,817.00
NextSeq2000 (100 Cycles) flow cell (P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell (P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell (P3)	$5,426.00	$6,403.00	$6,783.00

GGBC has a Federal Work-Study program laboratory position open for Fall 2021.

on May 26, 2021

GGBC has a Federal Work-Study program laboratory position open for Fall 2021. To apply go to handshake at https://uga.joinhandshake.com/login and search for position #4856258. More information about the FWS program and eligibility can be found at https://osfa.uga.edu/types-of-aid/undergraduate/federal_work-study/.

Due to the rapidly developing situation with the coronavirus pandemic and the UGA measures to effectively implement social distancing, the GGBC lab is closed at this time. However, the team is working remotely from homes to offer consultation on projects, write grant support letters, offer data analysis, provide cost estimates and quotes for new projects, etc. Please email us at ggbc@uga.edu and we will get back to you.

on March 19, 2020

GGBC Operation in the Time of Covid-19… click here

on March 16, 2020

A notice outlines Covid-19 lab operation changes: no in-person user communications, restricted access to GGBC members, sample drop-off instructions, and remote work for staff.

Category: Uncategorized

MiSeq and NextSeq Flow Cells