Sequel II System: high-throughput, cost-effective access to SMRT Sequencing
The Sequel System is based on Single Molecule, Real-Time (SMRT) technology and is ideal for rapidly and cost-effectively generating high-quality PacBio whole genome de novo assemblies and full-length transcriptomes. At GGBC, we provide all Sequel based services, from library prep and sequencing to data analysis.
PacBio Sequel II sequencing modes:
CLR (Continuous Long Read)
With reads tens of kilobases in length you can readily assemble complete genomes and sequence full-length transcripts. SMRT Sequencing provides exceptional read lengths without compromising throughput or accuracy. Traditional PacBio long reads are generated by a single pass of DNA Polymerase around a circularized template, which allows for overall longer read lengths but does not provide the higher accuracy characteristic of HiFi reads.
CCS (Circular Consensus Sequences)
Using SMRT technology, highly accurate long reads known as High Fidelity reads (HiFi) are attainable with an accuracy of >99.9%. This method ensures both long read sequencing depth, and high accuracy. HiFi reads are the data produced using CCS. During library preparation, SMRTbell adapters are ligated to double-stranded DNA, thus circularizing the library. Primers and DNA polymerase are annealed and the circularized library is sequenced in repeated passes of the enzyme around the template. Consensus is called from the multiple subreads to provide a highly accurate long HiFi read. Click here for more information on CCS.
Please see the Application section below for more information.
About Sequel II:
- Generates ~8-times more data than the original Sequel System (80Gbp +/- 20%)
- Provides access to even more highly accurate long reads (HiFi Reads)
- Reduces project time for faster results
- Makes sequencing more affordable
- Supports the range of SMRT Sequencing applications