June 2023 - GGBC

MiSeq and NextSeq Flow Cells

slquinlan on June 6, 2023

Table 4. MiSeq flow cells

Run Type	Expected single/paired-end reads passing filter	Expected Output	UGA Fee	Non-UGA Fee	Commercial Fee
v2 Flow Cell
300 cycles Nano	1M / 2M	300 Mb	$538	$635	$673
500 cycles Nano	1M / 2M	500 Mb	$608	$718	$760
300 cycles Micro	4M / 8M	1.2 Gb	$715	$844	$894
50 cycles	12-15M / 24-30M	600-750 Mb	$1,165	$1,375	$1,457
300 cycles	12-15M / 24-30M	4.5-5.1 Gb	$1,439	$1,699	$1,799
500 cycles	12-15M / 24-30M	7.5-8.5 Gb	$1,594	$1,881	$1,993
v3 Flow Cell
150 cycles	22-25M / 44-50M	3.3-3.8 Gb	$1,273	$1,503	$1,592
600 cycles	22-25M / 44-50M	13.2-15 Gb	$2,018	$2,382	$2,523

Table 5. NextSeq 2000 flow cells

Run Type	Expected single/paired-end reads passing filter	Expected Output	UGA Fee	Non-UGA Fee	Commercial Fee
P1 Flow Cell
100 cycles	100M / 200M	10 Gb	$1,232.00	$1,454.00	$1,540.00
300 cycles	100M / 200M	30 Gb	$1,540.00	$1,818.00	$1,925.00
600 cycles	100M / 200M	60 Gb	$2,113.00	$2,494.00	$2,642.00
P2 Flow Cell
100 cycles	400M / 800M	40 Gb	$1,721.00	$2,031.00	$2,152.00
200 cycles	400M / 800M	80 Gb	$2,850.00	$3,363.00	$3,563.00
300 cycles	400M / 800M	120 Gb	$3,636.00	$4,291.00	$4,545.00
600 cycles	400M / 800M	240 Gb	$3,920.00	$4,626.00	$4,900.00
P3 Flow Cell
50 cycles	1.2B / 2.4 B	60 Gb	$2,471.00	$2,916.00	$3,089.00
100 cycles	1.2B / 2.4 B	120 Gb	$3,374.00	$3,982.00	$4,218.00
200 cycles	1.2B / 2.4 B	240 Gb	$4,504.00	$5,315.00	$5,630.00
300 cycles	1.2B / 2.4 B	360 Gb	$5,859.00	$6,914.00	$7,324.00
P4 Flow Cell
50 cycles	1.8B/3.6B	90 Gb	$2,448.00	$2,889.00	$3,060.00
100 cycles	1.8B/3.6B	180 Gb	$3,453.00	$4,075.00	$4,317.00
200 cycles	1.8B/3.6B	360 Gb	$4,701.00	$5,548.00	$5,877.00
300 cycles	1.8B/3.6B	540 Gb	$5,693.00	$6,718.00	$7,117.00

Library Pooling and Quality Control

slquinlan on June 6, 2023

Table 3. Library pooling and Quality control

Service Description	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples	$63	$74.50	$78.75
Library Pooling up to 25-48 samples	$110	$130	$138
Library Pooling up to 49-96 samples	$170	$201	$213
Library Pooling up to 97-144 samples	$241	$285	$302
Library Pooling up to 145-192 samples	$268	$317	$335
Library Pooling up to 193-288 samples	$295	$349	$369
Library Pooling up to 289-384 samples	$378	$447	$473
Pre-Sequencing QC (Qubit, FA, Kapa)	$126	$149	$158
Additional Qubit quantification (per sample)	$5.00	$5.90	$6.25
Additional Fragment Analyzer: High sensitivity NGS fragment analysis (per sample)	$8.20	$9.70	$10.25
Additional Kapa qPCR (per sample)	$49.00	$57.85	$61.25

Illumina Library Preparation Costs

slquinlan on June 5, 2023

Table 1. Illumina library preparation costs NEW

Illumina Compatible Library Type	UGA Fee	UGA Fee	Non-UGA Fee	Non-UGA Fee	Commercial Fee	Commercial Fee
	_{(Price per library for up to 12)}	_{(Price for each additional library)}	_{(Price per library for up to 12)}	_{(Price for each additional library)}	_{(Price per library for up to 12)}	_{(Price for each additional library)}
RNA libraries
NGS Stranded (Illumina)	$80	$70	$94.50	$82.75	$100	$87.50
NGS Stranded (Roche KAPA)	$132	$112	$156	$133	$165	$140
NGS Ribo-Depleted Stranded or Unstranded RNA Library	Inquire	Inquire	Inquire	Inquire	Inquire	Inquire
NGS Small RNA	$115	$92	$136	$109	$144	$115
DNA libraries
NGS DNA	$45	$40	$53.10	$47.20	$56.25	$50
NGS DNA-PCR Free Library Prep	$128	$92	$152	$109	$160	$115
Enzymatic Methyl-Seq	$96	$96	$114	$114	$120	$120
Zymo-Seq RRBS	$120	$120	$142	$142	$150	$150

*Prices are significantly higher If libraries are to be prepared at GGBC but sequenced elsewhere. Please inquire for pricing.

Table 2. Illumina compatible library preparation reduced flat fees for samples submitted in a 96-well plate NEW

Illumina Compatible Library Type (submitted in 96 well, full or half plate)	UGA Fee	Non-UGA Fee	Commercial Fee
NGS Stranded RNA Full Plate Illumina Prep Kit	$4,800	$5,664	$6,000
NGS Stranded RNA Half Plate Illumina Prep Kit	$2,640	$3,116	$3,300
NGS Stranded RNA Full Plate: Roche KAPA Kit	$6,115	$7,216	$7,644
NGS Stranded RNA Half Plate: Roche KAPA Kit	$4,322	$5,100	$5,403
QIAseq FastSelect Ribo-depletion Full Plate	$5,125	$6,048	$6,407
QIAseq FastSelect Ribo-depletion Half Plate	$2,819	$3,326	$3,523
NGS DNA-PCR free	$7,639	$9,015	$9,549
NGS DNA Illumina: NGS DNA Library Prep (96-well full plate flat rate)	$1,920	$2,266	$2,400
NGS DNA Illumina: NGS DNA Library Prep (96-well half plate flat rate)	$1,200	$1,416	$1,500
Amplicons (16S/ITS/Custom)(49 to 96 samples per plate)	$949	$1,120	$1,187
Amplicons (16S/ITS/Custom)(up to 48 samples per plate)	$638	$753	$798
Amplicons (16S/ITS/Custom)(up to 24 samples per plate)	$360	$425	$450
Modified Amplicons (16S/ITS/Custom)(49 to 96 samples per plate)	$681	$804	$852
Modified Amplicons (16S/ITS/Custom)(up to 48 samples per plate)	$457	$540	$572
Modified Amplicons (16S/ITS/Custom)(up to 24 samples per plate)	$258	$305	$323

Extra charges will be incurred if sample(s) do not pass QC and are resubmitted. Any extra sample(s) above 96 will be charged according to Table 1.

**Illumina has changed the bead component in their library preparation kits and the Nextera DNA Flex products are now called Illumina DNA prep.

Immune Profiling

slquinlan on June 1, 2023

Table 7. 10x Genomics Immune Profiling 5’ GEX + V(D)J libraries preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$ 3,084.00	$ 3,640.00	$ 3,855.00
2	$ 5,346.00	$ 6,309.00	$ 6,683.00
3	$ 7,608.00	$ 8,978.00	$ 9,510.00
4	$ 9,871.00	$ 11,648.00	$ 12,339.00
5	$ 12,408.00	$ 14,642.00	$ 15,510.00
6	$ 14,670.00	$ 17,311.00	$ 18,338.00
7	$ 16,933.00	$ 19,981.00	$ 21,167.00
8	$ 19,195.00	$ 22,651.00	$ 23,994.00

Table 8. Suggested Illumina sequencing run type and associated fees for Immune Profiling 5’ GEX and V(D)J

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)(per run)	$120.00	$142.00	$150.00
NextSeq2000 (100 Cycles) flow cell(P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell(P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell(P3)	$5,426.00	$6,403.00	$6,783.00

Each Immune Profiling sample produces two libraries: 5’ GEX and V(D)J. Each library requires a separate Nextseq2000 sequencing run.

Single Cell Multiome ATAC + Gene Expression

slquinlan on June 1, 2023

Table 5. 10x Genomics Single Cell Multiome ATAC + Gene Expression libraries preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$4,345.00	$5.128.00	$5,432.00
2	$ 7,728.00	$ 9,120.00	$ 9,660.00
3	$11,111.00	$ 13,111.00	$ 13,889.00
4	$ 14,493.00	$ 17,102.00	$ 18,117.00
5	$ 18,151.00	$ 21,419.00	$ 22,689.00
6	$ 21,534.00	$ 25,411.00	$ 26,918.00
7	$ 24,917.00	$ 29,403.00	$ 31,147.00
8	$ 28,299.00	$ 33,393.00	$ 35,374.00

Table 6. Suggested Illumina sequencing run type and associated fees for Single Cell Multiome ATAC + Gene Expression libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)(per run)	$120.00	$142.00	$150.00
NextSeq2000 (100 Cycles) flow cell(P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell(P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell(P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell(P3)	$5,426.00	$6,403.00	$6,783.00

Each Multiome sample produces two libraries: ATAC and 3’RNAseq. Each library requires a separate NextSeq2000 sequencing run.

scATAC-seq

slquinlan on June 1, 2023

Table 3. 10x Genomics scATAC-seq library preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$2,759.00	$3,256.00	$3,449.00
2	$4,637.00	$5,472.00	$5,797.00
3	$6,515.00	$7,688.00	$8,144.00
4	$8,393.00	$9,904.00	$10,492.00
5	$10,537.00	$12,434.00	$13,172.00
6	$12,415.00	$14,650.00	$15,519.00
7	$14,293.00	$16,866.00	$17,867.00
8	$16,171.00	$19,082.00	$20,214.00

Table 4. Suggested Illumina sequencing run type and associated fees for scATAC-seq libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)	$120.00	$142.00	$150.00
NextSeq500 (150 Cycles) High Output flow cell	$3,899.00	$4,601.00	$4,874.00
NextSeq500 (75 Cycles) High Output flow cell	$2,253.00	$2,659.00	$2,817.00
NextSeq2000 (100 Cycles) flow cell (P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell (P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell (P3)	$5,426.00	$6,403.00	$6,783.00

Single Cell/Nuclei RNA seq

slquinlan on June 1, 2023

Table 1. 10x Genomics single cell/nuclei RNA seq library preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$2,758.00	$3,255.00	$3,448.00
2	$4,662.00	$5,502.00	$5,828.00
3	$6,565.00	$7,747.00	$8,207.00
4	$8,469.00	$9,994.00	$10,587.00
5	$10,622.00	$12,534.00	$13,278.00
6	$12,526.00	$14,781.00	$15,658.00
7	$14,429.00	$17,027.00	$18,037.00
8	$16,333.00	$19,273.00	$20,417.00

Table 2. Suggested Illumina sequencing run type and associated fees for single cell/nuclei RNAseq libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)	$120.00	$142.00	$150.00
NextSeq500 (150 Cycles) Mid Output flow cell	$1,763.00	$2,081.00	$2,204.00
NextSeq500 (150 Cycles) High Output flow cell	$3,899.00	$4,601.00	$4,874.00
NextSeq500 (75 Cycles) High Output flow cell	$2,253.00	$2,659.00	$2,817.00
NextSeq2000 (100 Cycles) flow cell (P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell (P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell (P3)	$5,426.00	$6,403.00	$6,783.00

Month: June 2023

MiSeq and NextSeq Flow Cells

Table 4. MiSeq flow cells

Table 5. NextSeq 2000 flow cells

Library Pooling and Quality Control

Table 3. Library pooling and Quality control

Illumina Library Preparation Costs

Table 1. Illumina library preparation costs NEW

Table 2. Illumina compatible library preparation reduced flat fees for samples submitted in a 96-well plate NEW

Immune Profiling

Table 7. 10x Genomics Immune Profiling 5’ GEX + V(D)J libraries preparation fees

Table 8. Suggested Illumina sequencing run type and associated fees for Immune Profiling 5’ GEX and V(D)J

Single Cell Multiome ATAC + Gene Expression

Table 5. 10x Genomics Single Cell Multiome ATAC + Gene Expression libraries preparation fees

Table 6. Suggested Illumina sequencing run type and associated fees for Single Cell Multiome ATAC + Gene Expression libraries

scATAC-seq

Table 3. 10x Genomics scATAC-seq library preparation fees

Table 4. Suggested Illumina sequencing run type and associated fees for scATAC-seq libraries

Single Cell/Nuclei RNA seq

Table 1. 10x Genomics single cell/nuclei RNA seq library preparation fees

Table 2. Suggested Illumina sequencing run type and associated fees for single cell/nuclei RNAseq libraries

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