LightCycler 480 System

The LightCycler® 480 System  is a compact and versatile benchtop real-time PCR platform that can hold multiwell plates for either 96 or 384 samples.

Lightcycler EquipmentThe LightCycler® 480 System is capable of running PCRs over a very broad range of volumes, ranging from 5 to 20 μl (384-well plate) and 20 to 100 μl (96-well plate). This allows for a highly flexible assay design, for example, when sensitivity is crucial because of low target concentrations.  Special multiwell plates to optimally fit the LightCycler®480 thermal block are an integral part of the system. They are used in combination with sealing foils that prevent evaporation and contamination. Bar codes on each plate allow easy sample tracking.

For fluorescence excitation, the LightCycler® 480 Instrument uses a high-intensity xenon lamp. The broad spectrum of this light source supports the use of a wide range of dyes and at the same time provides maximal sensitivity for a broad range of assay formats in all channels.

To make excitation as specific as possible and to reduce crosstalk between channels when more than one dye is used, the LightCycler® 480 Instrument includes filters (five different wavelength maxima – 440, 465, 498, 533 and 618 nm – for excitation; six wavelength maxima – 488, 510, 580, 610, 640, 660 nm – for detection). These filters can be flexibly combined according to the requirements of the probe format and dyes used in an experiment.

For signal capture, a large field lens and a charge-coupled device (CCD) camera are specifically arranged to attain uniform collection of signals across the plate. Fluorescence detection is therefore largely independent of sample position and can be done in the absence of additional dyes (e.g., ROX) used otherwise to compensate for two dimensional differences in intensity.

 

How to start a LightCycler Project at GGBC (Self-Service)

The LightCycler Real-Time PCR instrument can be used by any UGA customers after proper training and advanced scheduling.

  • Submit an order.
  •  Schedule an appointment to use the LightCycler by sending an e-mail to ggbc@uga.edu and referencing the order number in your request.
  •  Get an initial training on the instrument. A one-time training fee will be invoiced.
  •  Bring the samples to GGBC at the appointment time to proceed with your experiment.

     

Prices and Quotes

Contact for Financial Inquiries and Quote Requests

Please email Dr. Myriam Bélanger, Operations Manager, for financial inquiries or to request a quote. Be as specific as possible, so that she can more quickly assist you.

LightCycler Self-Service Fee
Initital training for UGA customers$50.00
Set up fee per order$5.50
Charge per minute for LightCyler usage$20.00/hr

For convenience, UGA customers can buy Roche white barcoded 96 or 384 well plates ($11 each) and Roche Sybr Green 1 tube (1 ml/100 rx; $86) from GGBC.