LightCycler 480 System
The LightCycler® 480 System is a compact and versatile benchtop real-time PCR platform that can hold multiwell plates for either 96 or 384 samples.
The LightCycler® 480 System is capable of running PCRs over a very broad range of volumes, ranging from 5 to 20 μl (384-well plate) and 20 to 100 μl (96-well plate). This allows for a highly flexible assay design, for example, when sensitivity is crucial because of low target concentrations. Special multiwell plates to optimally fit the LightCycler®480 thermal block are an integral part of the system. They are used in combination with sealing foils that prevent evaporation and contamination. Barcodes on each plate allow easy sample tracking.
For fluorescence excitation, the LightCycler® 480 Instrument uses a high-intensity xenon lamp. The broad spectrum of this light source supports the use of a wide range of dyes and at the same time provides maximal sensitivity for a broad range of assay formats in all channels.
To make excitation as specific as possible and to reduce crosstalk between channels when more than one dye is used, the LightCycler® 480 Instrument includes filters (five different wavelength maxima – 440, 465, 498, 533 and 618 nm – for excitation; six wavelength maxima – 488, 510, 580, 610, 640, 660 nm – for detection). These filters can be flexibly combined according to the requirements of the probe format and dyes used in an experiment.
For signal capture, a large field lens and a charge-coupled device (CCD) camera are specifically arranged to attain uniform collection of signals across the plate. Fluorescence detection is therefore largely independent of sample position and can be done in the absence of additional dyes (e.g., ROX) used otherwise to compensate for two dimensional differences in intensity.
- Perform genotyping experiments using HRMA, KASPar, TaqMan, etc.
- The LightCycler Real-Time PCR instrument can also be used by any UGA customers after proper training and advanced scheduling.
- The DNA should be purified with an optimized protocol, should not contain PCR inhibitors, and should appear intact when visualized by gel electrophoresis.
- The DNA should have a A260/280 ratio between 1.7 and 1.9.
- Nucleic acid purification is available from GGF.
- A *.csv or Excel file will need to be provided to GGF to keep track of the samples.
How to start a LightCycler Project with GGF
- There are two ways to proceed with a project:
- Self-Serve Service:
- 1) Submit an order. 2) Schedule an appointment to use the LightCycler by sending an e-mail to
- and referencing the order number in your request. 3) Get an initial training on the instrument. A one-time training fee will be invoiced. 4) Bring the samples to GGF at the appointment time to proceed with your experiment. 5) For invoicing purposes, record in the log book the number of minutes the instrument was in use.
- Project processed by GGF staff:
- 1) Contact the GGF staff at
- to discuss your project and submit an order. 2) Order the genotyping probes for the assay of choice. Once you receive your assay, please bring it to GGF with the samples to be tested. 3) Once done, data will be sent to the customer in tab delimited text and pdf format via e-mail. 4) Customers will be charged by GGF to run assays according to the Table found below.
|LightCycler Self-Service Fees|
|Initital training for UGA customers||$50.00|
|Set up fee per order||$5.30|
|Charge per minute for LightCyler usage||$20.00/hr|
For convenience, UGA customers can buy Roche white barcoded 96 or 384 well plates ($11 each) and Roche Sybr Green 1 tube (1 ml/100 rx; $86) from GGF.
Genotyping Assays (price per sample)
|Assay||UGA fee||Non-UGA fee||Commercial fee|
|High Resolution Melt Assays (per reaction)||$2.15||$2.55||$2.70|
|KASPar Set Up Fee (up to 96 samples)||$35.00||$41.30||$43.75|
|KASPar (per reaction)||$2.15||$2.55||$2.70|
Prices are subject to change without notice. QC, labor for dilutions and data analysis fees are not included in prices listed above. To get a project cost estimate or to get a quote for a grant proposal, please contact GGF.
- $60 per hour for UGA customers
- $90 per hour for non UGA customers
- $120 per hour for profit users