Capillary (Sanger) DNA Sequencing

The GGBC maintains an Applied Biosystems 3730xl 96-capillary DNA Analyzer for Image results for a SequencerSanger sequencing and fragment analysis. Users can submit 96 well plates of products that are ready to be sequenced or choose to do the cycle sequencing reaction themselves and we will process the “Ready-to-Run” plates on the 3730xl. Individual customer sequence read length will vary.

Customers may download data directly from their FBS Portal account. Upon request, data may be delivered via SFTP. Users are assigned password protected folders. All data resides within the PI’s folder, however independent subfolders can be obtained if requested.

 

 

Sample Preparation

Plate submissions for Sanger Sequencing

Please combine template DNA and primer using the following guidelines:

Template quantity required for sequencing

Purified Plasmid or Purified PCR Product (bp)Quantity Required (ng/µl)
100-2001-3
200-5003-10
500-10005-20
1000-200010-40
>200020-50
Double-stranded Plasmid150-300
*Cosmid, BAC*0.5-1.0 µg

Sequencing Primers

Primer concentrations 3.3µM

Per each reaction, use 1 ul primer (3.3µM)

 

Ready-to-run sequencing

Customers who want to do the cycle sequencing themselves can submit reactions in 96-well plates. Reactions should be submitted wet in high quality molecular grade water (if submitted dry, please let us know how much water to rehydrate them in).  The reactions have to be in semi-skirted plates that are compatible with the 3730xl.

If you are are submitting 24 or more samples, please submit samples in a 96-well plate and include an Excel file with the sample layout in your order.

PLEASE USE V BOTTOM PLATES AND NOT ROUND/FLAT BOTTOM PLATES.

Submit Samples

UGA and on-campus customers:

Please go to the FBS Portal page to submit your order and then bring your samples to the GGBC main office (Room 161) anytime Monday-Friday between 8:00 am-5:00 pm or leave them at one of our drop-off locations:

  • Coverdell Building, Coverdell Stockroom, freezer room on first floor (Get key from Room 146).
  • Chemistry Building, CSS, ABI fridge-bottom shelf.
  • Miller Plant Science Building, Room 4602 on 4th floor.
  • Life Science Building, Genetics Department, fridge is located in their office break room B103.
  • Poultry Diagnostic and Research Center (PDRC), 953 College Station Road, room 144.

All drop-off locations have spare envelopes in which your samples and primers can be submitted. Please do not tape your samples to your submissions sheet. Because of degradation issues, RNA samples must be brought directly to the GGBC main office.

Download a map of Drop off Locations (pdf)

Off-campus customers:

Please ship samples to this address:

University of Georgia
Georgia Genomics and Bioinformatics Core
110 Riverbend Rd, Rm 161
Athens, GA 30602

Tips: Please seal plates with plate sealing foil and ship on dry-ice or in freezer packs preferably through FedEx or UPS as they deliver directly to GGBC. You might also want to write “this side up” on your cooler or box.

Data Retrieval

Please go to the FBS Portal page to retrieve your data.

Prices and Quotes

Contact for Financial Inquiries and Quote Requests

Please email Kim and Elizabeth at ggbc@uga.edu, for financial inquiries or to request a quote. Be as specific as possible, so that they can more quickly assist you.

Current Price List

Service DescriptionUGA FeeNon-UGA FeeCommercial Fee
Ready to run genotyping plate$75.00$88.50$112.50
Ready to run sequencing plate$75.00$88.50$112.50
Sequencing a 96 well plate $260.00$307.00$390.00
Sephadex clean up/per plate$50.40$59.50$75.75
Sephadex clean up/per half plate$29.00$34.25$43.50
Semi-skirted 96-well plate (for 3730 sequencer)$19.00$22.45$28.50
Normalize DNA concentration in a 96 well plate$86.00$102.00$129.00

Prices are subject to change without notice. To get a  quote, please contact GGBC.

Data Analysis

Any of the following programs can be used to view DNA traces, but training and support are not available through GGBC .

Low-cost research data storage is available through the Georgia Advanced Computing Resource Center.

Capillary Sequencing Protocols