The GGF maintains an Applied Biosystems 3730xl 96-capillary DNA Analyzer for Sanger sequencing and fragment analysis.
Users can choose to submit individual DNA samples or 96 well plates of products that are ready to be sequenced or choose to do the cycle sequencing reaction themselves and we will process the “Ready-to-Run” plates on the 3730xl. Each instrument run contains a pGEM control that achieves a Q20 up to 650bp that is used to judge instrument performance. Individual customer sequence read length will vary.
All data are delivered via SFTP. Users are assigned password protected folders. All data resides within the PI’s folder, however independent subfolders can be obtained if requested.