DNA Size Selection

BluePippin: Sample Preparation and Submission Process

The BluePippin can be used to size select DNA fragments of interest during NGS library preparation. The selection ranges are:

  • 100 to 250 bp
  • 100 to 600 bp
  • 250 to 1,500 bp
  • 1 to 50 kb

Four samples can be run per pre-cast disposable agarose gel cassette. Submit a maximum of 5 µg of sheared genomic DNA per sample in 30 µl of DNA in TE. The minimum amount of sheared DNA that can be used is 15 ng.

More information on the BluePippin can be found on the Sage Science website.

BluePippin: Prices and Quotes

Contact for Financial Inquiries and Quote Requests

Please email Kim and Elizabeth at ggbc@uga.edu, for financial inquiries or to request a quote. Be as specific as possible, so that they can more quickly assist you.

Table 1. Price for DNA size selection on the BluePippin (up to 4 samples per cassette)

CustomerFee
UGA$108
Non-UGA$128
Commercial$135

The price of the run can be prorated if customers share a run. Otherwise, the full price as advertised above will be charged regardless of the number of samples submitted.

SageELF: Sample Preparation and Submission Process

The Sage Electrophoretic Lateral Fractionator, SageELF, is a gel electrophoresis system for automated separation and elution of DNA and proteins in 12 consecutive fractions of different sizes.

For DNA fractionation, three gel cassette types are available with the following target ranges:

  • 100 bp to 2.3 kb on 2% agarose gel (call GGBC to check availability)
  • 1 kb to 18 kb on 0.75% agarose gel (in stock)
  • 10 kb to 40 kb on 0.75% agarose gel (in stock)

Submit 100 ng to 5 µg of DNA in a maximum volume of 30 µL TE or nuclease free water. Samples should be free of ligases or polymerases and have low salt concentrations.

For protein fractionation, two gel cassettes types are available with the following target ranges:

  • 10 to 100 kDa on 5% agarose gel (call GGBC to check availability)
  • 50 to 500 kDa on 3% agarose gel (call GGBC to check availability)

More information on the SageELF can be found on the Sage Science website.

SageELF: Prices and Quotes

Contact for Financial Inquiries and Quote Requests

Please email Kim and Elizabeth at ggbc@uga.edu, for financial inquiries or to request a quote. Be as specific as possible, so that they can more quickly assist you.

Table 1. Price for DNA size selection on the SageELF (one sample per cassette)

CustomerFee
UGATBD
Non-UGATBD
CommercialTBD

Quality controls using the Qubit and Fragment Analyzer will be performed on eluted fractions (additional costs may be incurred).