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Table 4. MiSeq flow cells

Run Type	Expected single/paired-end reads passing filter	Expected Output	UGA Fee	Non-UGA Fee	Commercial Fee
v2 Flow Cell
300 cycles	12-15M / 24-30M	4.5-5.1 Gb	$1,771	$2,090	$2,214

Table 5. NextSeq 2000 flow cells

Run Type	Expected single/paired-end reads passing filter	Expected Output	UGA Fee	Non-UGA Fee	Commercial Fee
P1 Flow Cell
100 cycles	100M / 200M	10 Gb	$1,232.00	$1,454.00	$1,540.00
300 cycles	100M / 200M	30 Gb	$1,540.00	$1,818.00	$1,925.00
600 cycles	100M / 200M	60 Gb	$2,113.00	$2,494.00	$2,642.00
P2 Flow Cell
100 cycles	400M / 800M	40 Gb	$1,721.00	$2,031.00	$2,152.00
200 cycles	400M / 800M	80 Gb	$2,850.00	$3,363.00	$3,563.00
300 cycles	400M / 800M	120 Gb	$3,636.00	$4,291.00	$4,545.00
600 cycles	300M / 600M	180 Gb	$3,920.00	$4,626.00	$4,900.00
P3 Flow Cell
50 cycles	1.2B / 2.4 B	60 Gb	$2,471.00	$2,916.00	$3,089.00
100 cycles	1.2B / 2.4 B	120 Gb	$3,374.00	$3,982.00	$4,218.00
200 cycles	1.2B / 2.4 B	240 Gb	$4,504.00	$5,315.00	$5,630.00
300 cycles	1.2B / 2.4 B	360 Gb	$5,859.00	$6,914.00	$7,324.00
P4 Flow Cell
50 cycles	1.8B/3.6B	90 Gb	$2,554.00	$3,014.00	$3,193.00
100 cycles	1.8B/3.6B	180 Gb	$3,612.00	$4,263.00	$4,515.00
200 cycles	1.8B/3.6B	360 Gb	$4,925.00	$5,812.00	$6,157.00
300 cycles	1.8B/3.6B	540 Gb	$6,150.00	$7,257.00	$7,688.00

Library Pooling and Quality Control

slquinlan on June 6, 2023

Table 3. Library pooling and Quality control

Service Description	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples	$60	$71	$75
Library Pooling up to 25-48 samples	$105	$124	$132
Library Pooling up to 49-96 samples	$162	$192	$203
Library Pooling up to 97-144 samples	$234	$277	$293
Library Pooling up to 145-192 samples	$306	$362	$383
Library Pooling up to 193-288 samples	$436	$515	$545
Library Pooling up to 289-384 samples	$565	$667	$707
Pre-Sequencing QC (Qubit, FA, Kapa)	$120	$142	$150
Additional Qubit quantification (per sample)	$5.00	$5.90	$6.25
Additional Fragment Analyzer: High sensitivity NGS fragment analysis (per sample)	$8.20	$9.70	$10.25
Additional Kapa qPCR (per sample)	$49.00	$57.85	$61.25

Illumina Library Preparation Costs

slquinlan on June 5, 2023

Table 1. Illumina library preparation costs (prices valid for libraries prepared AND sequenced at GGBC ONLY*)

Illumina Compatible Library Type	UGA Fee	UGA Fee	Non-UGA Fee	Non-UGA Fee	Commercial Fee	Commercial Fee
	_{(Price per library for up to 12)}	_{(Price for each additional library)}	_{(Price per library for up to 12)}	_{(Price for each additional library)}	_{(Price per library for up to 12)}	_{(Price for each additional library)}
RNA libraries
NGS Stranded or Unstranded RNA (see Table 2)	$132	$112	$156	$133	$165	$140
NGS Ribo-Depleted Stranded or Unstranded RNA Library	$195	$195	$231	$231	$244	$244
NGS Small RNA	$115	$92	$136	$109	$144	$115
cDNA synthesis
cDNA 2nd strand synthesis only (1 to 24 samples per plate)	$447		$528		$559
cDNA 2nd strand synthesis only (25 to 48 samples per plate)	$894		$1,055		$1,118
cDNA 2nd strand synthesis only (49 to 72 samples per plate)	$1,341		$1,583		$1,677
cDNA 2nd strand synthesis only (73 to 96 samples per plate)	$1,788		$2,110		$2,235
Double stranded cDNA synthesis (1 to 24 samples per plate)	$598		$706		$748
Double stranded cDNA synthesis (25 to 48 samples per plate)	$1,195		$1,411		$1,494
Double stranded cDNA synthesis (49 to 72 samples per plate)	$1,793		$2,116		$2,242
Double stranded cDNA synthesis (73 to 96 samples per plate)	$2,390		$2,821		$2,988
cDNA from low input RNA – SMRT technology (Input 1-2 ng total RNA)	$159	$159	$188	$188	$239	$239
DNA libraries
NGS DNA ** (see Table 2)	$132	$95	$156	$113	$165	$119
NGS DNA-PCR Free Library Prep (1-12 samples)	$128		$152		$160
NGS DNA-PCR Free Library Prep (13 or more samples)	$92		$109		$115
Enzymatic Methyl-Seq	$96	$96	$114	$114	$120	$120
Zymo-Seq RRBS	$120	$120	$142	$142	$150	$150

*Prices are significantly higher If libraries are to be prepared at GGBC but sequenced elsewhere. Please inquire for pricing.

Table 2. Illumina compatible library preparation reduced flat fees for samples submitted in a 96-well plate

llumina Compatible Library Type (submitted in 96 well plate)	UGA Fee	Non-UGA Fee	Commercial Fee
NGS Stranded or Unstranded RNA	$6,115	$7,216	$7,644
TruSeq Stranded Total RNA Gold (Human/Rat/Mouse) (up to 48 samples per plate)	$8,810	10,396	$11,013
TruSeq Stranded Total RNA Gold (Human/Rat/Mouse) (49-96 samples per plate)	$15,537	$18,334	$19,422
QIAseq FastSelect Ribo-depletion 5S/16S/23S (96-well plate flat rate)	$5,125	$6,048	$6,407
NGS DNA-PCR free	$7,639	$9,015	$9,549
NGS DNA **	$7,830	$9,240	$9,788
Nextera Flex for Enrichment (up to 48 samples per plate)	$7,019	$8,283	$8,774
Nextera Flex for Enrichment (49-96 samples per plate)	$11,697	$13,803	$14,662
Modified Amplicons (16S/ITS/Custom)(up to 48 samples per plate)	$457	$540	$572
Modified Amplicons (16S/ITS/Custom)(49 to 96 samples per plate)	$681	$804	$852

Extra charges will be incurred if sample(s) do not pass QC and are resubmitted. Any extra sample(s) above 96 will be charged according to Table 1.

**Illumina has changed the bead component in their library preparation kits and the Nextera DNA Flex products are now called Illumina DNA prep.

Table 7. 10x Genomics Immune Profiling 5’ GEX + V(D)J libraries preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$ 3,084.00	$ 3,640.00	$ 3,855.00
2	$ 5,346.00	$ 6,309.00	$ 6,683.00
3	$ 7,608.00	$ 8,978.00	$ 9,510.00
4	$ 9,871.00	$ 11,648.00	$ 12,339.00
5	$ 12,408.00	$ 14,642.00	$ 15,510.00
6	$ 14,670.00	$ 17,311.00	$ 18,338.00
7	$ 16,933.00	$ 19,981.00	$ 21,167.00
8	$ 19,195.00	$ 22,651.00	$ 23,994.00

Table 8. Suggested Illumina sequencing run type and associated fees for Immune Profiling 5’ GEX and V(D)J

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)(per run)	$120.00	$142.00	$150.00
NextSeq2000 (100 Cycles) flow cell(P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell(P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell(P3)	$5,426.00	$6,403.00	$6,783.00

Each Immune Profiling sample produces two libraries: 5’ GEX and V(D)J. Each library requires a separate Nextseq2000 sequencing run.

Single Cell Multiome ATAC + Gene Expression

slquinlan on June 1, 2023

Table 5. 10x Genomics Single Cell Multiome ATAC + Gene Expression libraries preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$4,345.00	$5.128.00	$5,432.00
2	$ 7,728.00	$ 9,120.00	$ 9,660.00
3	$11,111.00	$ 13,111.00	$ 13,889.00
4	$ 14,493.00	$ 17,102.00	$ 18,117.00
5	$ 18,151.00	$ 21,419.00	$ 22,689.00
6	$ 21,534.00	$ 25,411.00	$ 26,918.00
7	$ 24,917.00	$ 29,403.00	$ 31,147.00
8	$ 28,299.00	$ 33,393.00	$ 35,374.00

Table 6. Suggested Illumina sequencing run type and associated fees for Single Cell Multiome ATAC + Gene Expression libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)(per run)	$120.00	$142.00	$150.00
NextSeq2000 (100 Cycles) flow cell(P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell(P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell(P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell(P3)	$5,426.00	$6,403.00	$6,783.00

Each Multiome sample produces two libraries: ATAC and 3’RNAseq. Each library requires a separate NextSeq2000 sequencing run.

Table 3. 10x Genomics scATAC-seq library preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$2,759.00	$3,256.00	$3,449.00
2	$4,637.00	$5,472.00	$5,797.00
3	$6,515.00	$7,688.00	$8,144.00
4	$8,393.00	$9,904.00	$10,492.00
5	$10,537.00	$12,434.00	$13,172.00
6	$12,415.00	$14,650.00	$15,519.00
7	$14,293.00	$16,866.00	$17,867.00
8	$16,171.00	$19,082.00	$20,214.00

Table 4. Suggested Illumina sequencing run type and associated fees for scATAC-seq libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)	$120.00	$142.00	$150.00
NextSeq500 (150 Cycles) High Output flow cell	$3,899.00	$4,601.00	$4,874.00
NextSeq500 (75 Cycles) High Output flow cell	$2,253.00	$2,659.00	$2,817.00
NextSeq2000 (100 Cycles) flow cell (P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell (P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell (P3)	$5,426.00	$6,403.00	$6,783.00

Table 1. 10x Genomics single cell/nuclei RNA seq library preparation fees

Number of Sample(s)	UGA Fee	Non-UGA Fee	Commercial Fee
1	$2,758.00	$3,255.00	$3,448.00
2	$4,662.00	$5,502.00	$5,828.00
3	$6,565.00	$7,747.00	$8,207.00
4	$8,469.00	$9,994.00	$10,587.00
5	$10,622.00	$12,534.00	$13,278.00
6	$12,526.00	$14,781.00	$15,658.00
7	$14,429.00	$17,027.00	$18,037.00
8	$16,333.00	$19,273.00	$20,417.00

Table 2. Suggested Illumina sequencing run type and associated fees for single cell/nuclei RNAseq libraries

Service Description(s)	UGA Fee	Non-UGA Fee	Commercial Fee
Library Pooling up to 2-24 samples*	$60	$71	$75
Pre-Sequencing QC (Qubit, FA, Kapa)	$120.00	$142.00	$150.00
NextSeq500 (150 Cycles) Mid Output flow cell	$1,763.00	$2,081.00	$2,204.00
NextSeq500 (150 Cycles) High Output flow cell	$3,899.00	$4,601.00	$4,874.00
NextSeq500 (75 Cycles) High Output flow cell	$2,253.00	$2,659.00	$2,817.00
NextSeq2000 (100 Cycles) flow cell (P2)	$2,013.00	$2,376.00	$2,517.00
NextSeq2000 (200 Cycles) flow cell (P2)	$3,398.00	$4,010.00	$4,248.00
NextSeq2000 (100 Cycles) flow cell (P3)	$4,040.00	$4,768.00	$5,050.00
NextSeq2000 (200 Cycles) flow cell (P3)	$5,426.00	$6,403.00	$6,783.00

Join Us: PacBio Genomics Day – Thursday, October 13, 2022, 8:00am to 1:00pm

eashwort on September 30, 2022

Here is the registration page: https://events.pacb.com/pacbiogenomicsday-atlanta/home

GGBC has a Federal Work-Study program laboratory position open for Fall 2021.

eashwort on May 26, 2021

GGBC has a Federal Work-Study program laboratory position open for Fall 2021. To apply go to handshake at https://uga.joinhandshake.com/login and search for position #4856258. More information about the FWS program and eligibility can be found at https://osfa.uga.edu/types-of-aid/undergraduate/federal_work-study/.

Due to the rapidly developing situation with the coronavirus pandemic and the UGA measures to effectively implement social distancing, the GGBC lab is closed at this time. However, the team is working remotely from homes to offer consultation on projects, write grant support letters, offer data analysis, provide cost estimates and quotes for new projects, etc. Please email us at ggbc@uga.edu and we will get back to you.

eashwort on March 19, 2020

Category: Uncategorized

MiSeq and NextSeq Flow Cells